Lab Report on Urine for AP II Essay Example

Lab Report on Urine for AP II Paper This situation normally arises in diabetes patients. In renal diabetes, the renal threshold is reduced to the point where sugar appears in the urine despite normal levels being present in the blood. In both situations, monitoring of urine glucose becomes important. In the following exercises it will be determined, by using three simulated urine specimens, what color, pH, specific gravity, glucose, and protein has on urine. Urine Normal is normal, and Urine high (positive) on all tests. In part 2, unknown simulated urine will be tested and determine the probable disorders. Materials and Methods: Part 1 Color: Record the color of each simulated urine (Low, Normal, and High) in Table pH: 1. Obtain three pH indicator strips and label each to match the containers 2. Test each simulated urine (Low, Normal, and High) by dipping the appropriate pH strip into it three consecutive times. 3. Shake off excess liquid and compare the color of the strip to the colors on the pH chart. The color that most closely matches your strip corresponds to the pH of the simulated urine. 4. Record each pH in Table 1. The pH of normal urine averages 6. , which is slightly acidic. Certain foods and diseases that can affect urine PH. Specific Gravity . The instructor will either instruct you to calibrate the hydrometer or provide you with a calibration factor. Record the calibration factor in Table 1. 2. Obtain the urine hydrometer and jar and rinse both pieces well. Fill the jar three-fourths full of Urine Low. Place the hydrometer in the jar so that it is not touching the sides a nd read the level of urine on the hydrometer scale. Record the measured (base) urine specific gravity in Table 1 . 3. We will write a custom essay sample on Lab Report on Urine for AP II specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Lab Report on Urine for AP II specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Lab Report on Urine for AP II specifically for you FOR ONLY $16.38 $13.9/page Hire Writer The instructor will give instructions on whether to add or subtract from the reading of the calibration factor. Record the adjusted urine specific gravity in Table 1. 4. Remove the hydrometer and pour the simulated urine sample back into the container. 5. Rinse the hydrometer and jar well. 6. Repeat steps 2 to 5 with Urine Normal and Urine High. Specific gravity varies according to fluid intake and can also be affected by diseases. Glucose 1. Using a dropping pipette, place 5 drops of urine into a glass vial. 2. Rinse the dropper thoroughly with water and add 10 drops of water to the vial. . Drop one [emailprotected] tablet into the vial. Place the vial on the tabletop and observe the reaction. Caution: the glass vial will become very hot during the chemical reaction. 4. After the reaction has stopped, wait 15 seconds. Shake gently to mix the contents. Caution: do not allow the contents of the vial to come into contact with your skin or eyes. Compare the color to the [emailprotected] color chart. 5. Record the results of the test for glucose (positive or negative) in Table 1 . Glucose (sugar) should not be detected in normal urine. The presence of glucose usually indicates diabetes mellitus, a severe metabolic disorder due to defective carbohydrate utilization. Protein 1. Using a 10 ml graduated cylinder, add 1 ml urine too clean glass vial. 2. Rinse the graduated cylinder and measure 2 ml Beirut reagent. Note the pale blue color of the Beirut reagent. 3. Add the Beirut reagent to the urine vial. 4. Gently swirl the vial to mix the contents. After 10 minutes hold the test tube against a white background and observe the color. A color change from light blue to a pale violet indicates the presence of protein. . Record the results of the test for protein (positive or negative) in Table 1. Normally, protein should not be detected in the urine. Results: Urine Test Low Normal High Urine A Urine B Color Light Yellow Light Yellow Clear (White) Clear (no color) Pink pH37858 Specific gravity: Base 1. 02 1. 025 1. 029 1. 032 1. 034 Glucose Negative Negative Present Present Negative Protein Negative Negative Negative Negat ive Positive Table 1 Discussion: From the above data, it can be determined that not all of the urinalysis results were normal.